European Psychiatry - Volume 41 - April 2017

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25th European Congress of Psychiatry / European Psychiatry 41S (2017) S521–S582 S551
Methods A total of 806 ED patients (792 females; 379 AN and
427 BN) were recruited. Diagnosis of AN and BN were ascertained
according to the DSM-IV-TR criteria by means of the SCID – Patient
Edition. AAO was assessed by a clinical interview performed by a
psychiatrist matched with a systematic review of medical records.
To test AAO subgroups, we used a normal distribution admixture
analysis.
Results A bimodal normal distribution of AAOwith an early onset
and late onset component was found for both AN and BN. Most of
the subjects with AN (75.3%) and BN (83.3%) belonged to the early
onset group. Both groups had a mean AAO of about 18 years. No
significant differences were found concerning the AAO between
groups.
Conclusion Consider clinical history and course of AAO for EDs
may be crucial for planning treatment. To our knowledge, this is the
first study that applied a validated statistical procedure to identify
AAO cut-off points for AN and BN.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
EV0451
Interhemispheric functional
connectivity in anorexia and bulimia
nervosa
R. Amodio ∗, A. Prinster , A.M. Monteleone , F. Esposito ,
A. Canna , E. Cantone , U. Volpe , P. Monteleone
1 Second University of Naples, Department of Psychiatry, Naples, Italy
2 National Research Council, Biostructure and Bioimaging Institute,
Naples, Italy
3 University of Salerno, Department of Medicine and Surgery, Salerno,
Italy
4 University Federico II of Naples, ENT Department, Naples, Italy
∗ Corresponding author.
Introduction The functional interplay between brain hemi-
spheres is fundamental for behavioral, cognitive and emotional
control. Several pathophysiological aspects of eating disorders
(EDs) have been investigated by the use of functional Magnetic
Resonance Imaging (fMRI).
Objectives The objective of the study was to investigate func-
tional brain asymmetry of resting-state fMRI correlations in
symptomatic patients with anorexia nervosa (AN) and bulimia ner-
vosa (BN).
Aims We aimed at revealing whether brain regions implicated in
reward, cognitive control, starvation and emotion regulation show
altered inter-hemispheric functional connectivity in patients with
AN and BN.
Methods Using resting-state fMRI, voxel-mirrored homotopic
connectivity (VMHC) and regional inter-hemispheric spectral
coherence (IHSC) analyses in two canonical slow frequency bands
(“Slow-5”, “Slow-4”) were studied in 15AN and 13BN patients and
16 healthy controls (HC). Using T1-weighted and diffusion tensor
imaging MRI scans, regional VMHC values were correlated with the
left-right asymmetry of corresponding homotopic gray matter vol-
umes and with the white matter callosal fractional anisotropy (FA).
Results Compared to HC, AN patients exhibited reduced
VMHC in cerebellum, insula and precuneus, while BN patients
showed reduced VMHC in dorso-lateral prefrontal and orbito-
frontal cortices. The regional IHSC analysis highlighted that the
inter-hemispheric functional connectivity was higher in the ‘Slow-
5 Band in all regions except the insula. No group differences in
left-right structural asymmetries and in VMHC vs callosal FA cor-
relations were found.
Conclusions These anomalies indicate that AN and BN, at least in
their acute phase, are associated with a loss of inter-hemispheric
connectivity in regions implicated in self-referential, cognitive con-
trol and reward processing.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
EV0452
Investigation of endocannabinoids
and endocannabinoid-related
compounds in obese subjects during
an hedonic eating experimental test
V. Ruzzi ∗, A.M. Monteleone , F. Monaco , G. Fico , A. Cervino ,
U. Volpe , P. Monteleone
1 Second University of Naples, Department of Psychiatry, Naples, Italy
2 University of Salerno, Department of Medicine and Surgery, Salerno,
Italy
∗ Corresponding author.
Introduction Hedonic eating refers to the consumption of food
just for pleasure and not for energetic needs. Endocannabinoids
and endocannabinoid-related compounds play an important role
in food-related reward and are likely involved in hedonic eating.
Objectives In a previous study we found that in normal weight
healthy subjects plasma levels of 2 arachidonoylglycerol (2-AG)
decreased progressively after food ingestion in both hedonic
and non-hedonic eating condition, but they were significantly
higher in hedonic eating. Plasma levels of anandamide (AEA),
oleoylethanolamide (OEA) and palmitoylethanolamide (PEA),
instead, progressively decreased in both eating conditions without
significant differences.
Aims In order to investigate the physiology of endocannabinoids
in obesity, we assessed the responses of AEA, 2-AG, OEA and PEA
to hedonic and non-hedonic eating in obese individuals.
Methods Fourteen satiated obese patients consumed favorite
(hedonic eating) and non-favorite (non-hedonic eating) foods in
two experimental sessions. During the tests, blood was collected to
measure peripheral levels of AEA, 2-AG, OEA and PEA.
Results Plasma levels of 2-AG progressively decreased in non-
hedonic eating whereas they gradually increased after hedonic
eating. Plasma levels of AEA decreased progressively in non-
hedonic eating, whereas they initially decreased after the
exposure to the favorite food and then returned to base-
line values after its consumption. The responses of OEA and
PEA to favorite and non-favorite food did not show significant
differences.
Conclusions These findings demonstrate that, compared to nor-
mal weight healthy subjects, obese subjects exhibit different
responses of peripheral endocannabinoids to the ingestion of
food for pleasure and this could have implications for the
onset/maintenance of obesity.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
EV0453
Attachment and
hypothalamus-pituitary-adrenal axis
functioning in patients with eating
disorders
F. Monaco ∗, A.M. Monteleone , F. Pellegrino , V. Ruzzi ,
G. Fico , U. Volpe , P. Monteleone
1 Second University of Naples, Department of Psychiatry, Naples, Italy
2 University of Salerno, Department of Medicine and Surgery, Salerno,
Italy
∗ Corresponding author.
Introduction Attachment theory suggests that different attach-
ment styles influence the development of individual’s self-esteem
and modulate the individual’s ability to manage stressful events