European Psychiatry - Volume 41

S464

25th European Congress of Psychiatry / European Psychiatry 41S (2017) S405–S464

diseases. Human mitochondria with its own genetic material meet

the needs required for the assembly of subunits of the oxidative

phosphorylation (OXPHOS) complexes. A number of translational

inhibitors are known that could potentially effect translation of

mitochondrial protein synthesis. Among these puromycin, homo-

harringtonine and cyclohexamide were selected for the present

study. The effect of these translational inhibitors on mitochon-

drial gene expression for the treatment of neuroblastoma are not

well established. Therefore, in this study, we have investigated

the effects of these translational inhibitors on the expression of

human mitochondrial gene expression in SH-SY5Y neuroblastoma

cells.

We observed a significant effect on the level of mitochondrial trans-

cripts upon exposure to these translation inhibitors in SH-SY5Y

cells, however, the effects on expression of mitochondrial proteins

were minimal. This suggests that translational inhibitors might not

directly affect the abundance of mitochondrial proteins. Transla-

tional inhibitors induce significant effect on mitochondrial gene

expression that can be lead to the new-targeted therapy for treating

neuroblastoma.

Disclosure of interest

The author has not supplied his declaration

of competing interest.