European Psychiatry - Volume 41 - April 2017

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S102 25th European Congress of Psychiatry / European Psychiatry 41S (2017) S69–S105
response to CYP2D6 substrates comparing to wild type homozy-
gous. As none of the analyzed patients was PM, exceeded plasma
concentrations of medications above toxic levels are not expected
when administrating the right dosage.
Conclusion Altered CYP2D6 metabolism may contribute to
the vulnerability, clinical severity and treatment outcome of
schizophrenia.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
O093
Differential susceptibility properties
of the 5HTTLPR gene in relation to
depressive symptoms and
delinquency
K.-W. Nilsson ∗, C. Åslund
Uppsala University, Centre for clinical research, Västerås, Sweden
∗ Corresponding author.
Introduction The candidate gene-environment interaction
(cG ×E) research field in psychiatry has traditionally been domi-
nated by the diathesis–stress framework, where certain genotypes
are assumed to confer increased risk for adverse outcomes in
a stressful environment. In later years, theories of differential
susceptibility or biological sensitivity have been presented, sug-
gesting that cGs that interact with environmental events do not
exclusively confer a risk for behavioural or psychiatric disorders
but rather seem to alter the sensitivity to both positive and
negative environmental influences.
Aims The present study investigates the susceptibility properties
of the 5HTTLPR gene in relation to depressive symptoms and delin-
quency in two separate adolescent community samples: n = 1457,
collected in 2006; and n = 191, collected in 2001.
Results Two-, three- and four-way interactions between the
5HTTLPR , positive family environment, negative family environ-
ment, and sex were found in relation to both depressive symptoms
and delinquency. However, the susceptibility properties of the
5HTTLPR genewere distinctly less pronounced in relation to depres-
sive symptoms.
Conclusions If the assumption that the 5HTTLPR gene induces dif-
ferential susceptibility to both positive and negative environmental
influences is correct, the previous failures to measure and control
for positive environmental factors might be a possible explanation
for former inconsistent findings within the research field.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
O094
Epigenetics in the remission of
anorexia nervosa: A follow-up study of
whole-genome methylation profiles
N. Ramoz ∗, S. Guillaume , P. Courtet , P. Gorwood
1 Inserm U894, Center of Psychiatry and Neuroscience, Paris, France
2 CHU de Montpellier, hôpital Lapeyronie–Psychiatry, Montpellier,
France
3 Inserm, U1061, Montpellier, France
4 Hôpital Sainte-Anne, CMME, Paris, France
∗ Corresponding author.
Introduction Anorexia nervosa (AN) is a severe psychiatric disor-
der. The epigenetic regulations are strongly suggested in AN. We
and other groups have performed a whole-genome methylation
study (methylome) in AN. We found that the differentially meth-
ylated CpG sites are located around genes involved in biological
processes in link with embryonic morphogenesis, brain develop-
ment and its plasticity, in particular adhesion and axon guidance.
Here, we study an independent group of 40 AN patients. Further-
more, we have done a follow-up during more than one year, to
compare the methylation profiles in subjects that evolve to the
remission.
Objectives Our work is to replicate the methylome study in an
independent AN cohort and to characterize profiles of methylation
at two times for the same subjects to compare the AN patients that
convert to remitters.
Aims Our goal is to identify diagnostic and prognostic epigenetic
signatures for AN.
Methods Of the 40 AN patients, 18 evolved to remission. Further-
more, the blood samples of the subjects from the 2 times will be
investigated, like this, each subject is its own control. Methylation
of DNA is measured by using the Infinium HumanMethylation450
BeadChip technology.
Results Comparisons of AN to controls showed similar profiles of
methylation involving the same biological processes as previously
identified. We are comparing now the difference of methylation
between the 18 remitters and the 18 actual AN, taking into account
of the two times of samples.
Conclusions We expect to characterize specific methylation sig-
nature of the prognostic of the AN remission.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
O095
Exploring lithium impact on
glomerular function in bipolar
patients through pharmacogenomics
E.E. Tsermpini ∗, Y. Zhang , P. Niola , C. Chillotti , R. Ardau ,
G. Severino , A. Bocchetta , G.P. Patrinos , M.T.M. Lee ,
A. Squassina
1 University of Patras, School of Health Science, Department of
Pharmacy, Laboratory of Molecular Biology and Immunology, Patras,
Greece
2 RIKEN Center for Integrative Medical Sciences, Laboratory for
International Alliance on Genomic Research, Yokohama, Japan
3 University of Cagliari, Section of Neurosciences and Clinical
Pharmacology, Department of Biomedical Sciences, School of
Medicine, Cagliari, Italy
4 University Hospital of Cagliari, Unit of Clinical Pharmacology,
Cagliari, Italy
∗ Corresponding author.
Introduction Bipolar disorder (BD) is characterized by unusual
shifts in mood and energy and affects 1 to 3% of the general popula-
tion. Lithium (Li) can prevent patients from depression and mania,
as well as reduce the risk of suicide. Unfortunately, a high rate of
patients do not respond positively to Li treatment. In line with var-
ious studies, Li treatment is also associated with potentially severe
adverse reactions, including renal dysfunctions. Specifically, it has
been reported that Li may induce reduction of glomerular filtration
rate (GFR) in long-term treated BD patients.
Aims The aim of our study was to evaluate the contribution
of genetic variants in Li-induced reduction of the estimated GFR
(eGFR) in bipolar patients, under long term Li therapy.
Objectives We screened the literature to identify genes previ-
ously shown to be associatedwith kidney function or Li mechanism
of action and genotyped tag SNPs covering these genes.
Methods The sample comprised 70 Sardinian bipolar patients
genotyped for 46 SNPs, located in 33 genes, with Invader assay and
Sanger sequencing.
Results Our results showed that a SNP (rs378448) located in
Acid Sensing Ion Channel Neurona-1 (ACCN1) gene, significantly
interacted with years of Li treatment in reducing eGFR (F = 4.166,
P = 0.046).