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25th European Congress of Psychiatry / European Psychiatry 41S (2017) S303–S364


N. Smaoui , N. Charfi

, M. Turki , M. Maâlej-Bouali , L. Zouari ,

N. Zouari , J. Ben Thabet , M. Maâlej

Hedi Chaker university hospital, department of psychiatry C, Hedi

Chaker university hospital, Sfax, Tunisia

Corresponding author.


It is widely recognized that parents and peers play

a critical role in the adolescent’s introduction to alcohol.


The aim of the study was to examine the relationship

of parental and peers drinking to adolescent drinking behavior.


A cross-sectional study was carried out in four colleges

and schools in Sfax in Tunisia, in May and June 2016. The sample

consisted of 317 pupils, and was determined through a simple

randomized sampling. These adolescents were asked to answer a

self-administered questionnaire, after their consent. Alcohol use

disorders identification test (AUDIT) was used to evaluate alcohol



The mean age was 16 years, with a sex-ratio of 1.07.

The participants reported having drunk alcohol at least once in

18.9% of cases and 41.66% of them still consume. According to

AUDIT, 1.6% of alcohol users presented an alcohol misuse and

21.6% presented dependence. They reported that parents’ atti-

tude toward their alcohol use was favorable in 27.11% of cases.

Among dependent adolescents, the prevalence of fathers’ alcohol

consumption was 20% while that of friends was 70%. Adolescent

drinking was significantly correlated to fathers, mothers and peers

drinking (


< 0.001,


= 0.004,


< 0.001 respectively), mothers and

peers smoking (


= 0.05,


< 0.001 respectively), fathers and peer’s

cannabis use (


< 0.001,


< 0.001 respectively).


Findings suggest that negative family and peers

influence increased risk of alcohol consumption in adolescents.

Understanding the influences on parents’ beliefs about their chil-

dren’s drinking and the functions of social networks in preventing

alcohol consumption may be necessary to address adolescent risky


Disclosure of interest

The authors have not supplied their decla-

ration of competing interest.


Drug metabolizing enzyme and

transporter genes associated with

plasma risperidone level in Thai

autism spectrum disorder

C. Sukasem

Faculty of medicine Ramathibodi hospital, Pathology, Bangkok,



The associations between genetic variants of drug

metabolizing enzyme and transporter (DMET) genes and steady-

state plasma concentrations of risperidone, 9-hydroxyrisperidone,

total active-moiety, and metabolic ratio remain unclear.


The objective of the present study was to present the

results of the association between genetic variants of DMET gene

and steady-state plasma concentration risperidone and its meta-

bolite using Affymetrix DMET Plus genotyping microarray.


Subjects eligible for this study included male and

female adolescents with ASD diagnosed according to the Dia-

gnostic and Statistical Manual of Mental Disorders, Fourth Edition

(DSM-IV) criteria and being treated with risperidone for at least

4 weeks prior to the blood sample collection. Blood samples

were drawn prior to the next dose of risperidone intake to

determine the steady-state plasma trough concentrations of

risperidone and 9-hydroxyrisperidone. Genotyping profile was

obtained using the microarray. Steady-state plasma risperidone

and 9-hydroxyrisperidone were measured using liquid chromato-

graphy/tandem mass spectrometry (LC-MS/MS) assay.


The polymorphisms of UGT2B4, CYP2D6 were highly

associated with metabolic ratio. Of all the DMET analysis, ABCB11

(3084A > G, 420A > G, 368G > A, and 236G > A) and ADH7 (690G > A

and –5360G > A) were found to be associated with plasma concen-

trations of risperidone (


< 0.01). In addition, 6 genetic variations

among the SLC transporter family were associated with the plasma

concentration of 9-hydroxyrisperidone.


This study provides a pharmacogenomic approach

to investigate further among the DMET genetic variants which

influence plasma concentration of risperidone. The treatment of

ASD should be based on genetic factors making the challenge

of psychopharmacological treatment more efficacious with lesser

adverse events.

Disclosure of interest

The author has not supplied his/her decla-

ration of competing interest.


Exome sequencing detection of

genetic markers for Thai autism

spectrum disorder

C. Sukasem

Faculty of medicine Ramathibodi hospital, Pathology, Bangkok,



Autismspectrumdisorders (ASD) are neurodevelop-

mental disorders characterized by abnormalities in 3 domains;

social interaction, communication/language, and restricted and

repetitive behavior. The study of ASD prevalence in Thailand sho-

wed that it is approximately 9.9 children per 10,000 population for

children 1–5 years old. ASD has a strong genetic basis, although the

genetics of autism are complex and it is unclear. The objective of

this study was to identify the genetic markers of Thai ASD.


Exome sequencing was performed with twelve unre-

lated ASD affected individuals from twelve families. Each sample

was sequenced on SOLiD 5500xl genetic analyzer, and the resulting

data was processed and analyzed on LifeScope Genomic Analy-

sis software. Exome sequencing with two additional samples was

performed Ion Proton System and the data was processed on Ion

Reporter server. Tertiary data analysis with all fourteen exome

sequencing data were performed by using Golden Helix software.

In filtering process, datawere annotated to various databases inclu-

ding UCSC KnownGenes for non-coding and synonymous variants

filter, 1000 Genomes Project for high frequency variants filter, and

dbNSFP for functional prediction.


The genetic markers were identified for Thai ASD asso-

ciated variants (c.2014G > A in EIF2AK3, c.2951G > A in FGD6, and

c.6119A > G in CHD8).


these geneticmarkerswere themost possible of cau-

sing variants Thai. We also demonstrated a potential of exome

sequencing and bioinformatics pipeline to identify the possible

causative variants of ASD, which could by applied in the case that

unable to identified variants by other technique.

Disclosure of interest

The author has not supplied his/her decla-

ration of competing interest.


Hyperuricemia and metabolic adverse

effect in children and adolescents

with autism spectrum disorder

treated with risperidone

C. Sukasem

Faculty of medicine Ramathibodi hospital, Pathology, Bangkok,